Composite

Part:BBa_K4129004:Design

Designed by: Giovanni Carlo Gendarini   Group: iGEM22_DTU-Denmark   (2022-10-02)


mCherry cassette under the regulation of the promoter of the Saccharomyces cerevisiae FDH1 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1915
    Illegal BamHI site found at 2175
    Illegal XhoI site found at 278
    Illegal XhoI site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 193
    Illegal BsaI.rc site found at 345


Design Notes

BBa_K3046009 is designed to allow for its promoter to be switch using Golden Gate assembly. However, BBa_K4129000 is not compatible with Golden Gate as it contains BsaI sites. USER cloning was used to assemble the part instead.



Source

The part was obtained by inserting the scFDH1 promoter (BBa_K4129000) in place of the prokaryotic promoter of BBa_K3046009 by USER cloning.

References